美國(guó)Cyto Pulse Sciences, Inc.  www.cytopulse.com
美國(guó)Cyto Pulse Sciences, Inc.成立于1995年.是一家專(zhuān)業(yè)從事電穿孔、電融合儀器研發(fā)和生產(chǎn)的公司，多年來(lái)致力于研究用于醫(yī)療和商業(yè)用途的先進(jìn)脈沖電場(chǎng)技術(shù)系統(tǒng)，并將其成果應(yīng)用于科學(xué)研究。擁有獨(dú)一無(wú)二的 PulseAgile 協(xié)議方波、細(xì)胞融合和電穿孔專(zhuān)用培養(yǎng)基、控制軟件等多項(xiàng)技術(shù)。Cyto Pulse 能夠提供參數(shù)(強(qiáng)度、脈寬、方向、重復(fù)頻率等)更加快速、靈活變化的脈沖電場(chǎng)，從而實(shí)現(xiàn)高效的轉(zhuǎn)化和融合效率。

該公司與全球幾十家科研機(jī)構(gòu)建立了長(zhǎng)期合作，其產(chǎn)品接受了廣泛的測(cè)試和改進(jìn)，廣泛的應(yīng)用于動(dòng)植物細(xì)胞及微生物的轉(zhuǎn)化、雜交瘤、細(xì)胞融合等相關(guān)研究。
2010年法國(guó)Cellectis 收購(gòu)美國(guó)Cyto Pulse Sciences 

Cyto Pulse Sciences, Inc. was founded in December 1995 and is incorporated as a regular C corporation in Maryland. The company is composed of a multidisciplinary staff of engineers and scientists. Cyto Pulse develops electric field based devices for use in vaccine delivery, vaccine production, personal vaccine production, gene therapy, and hybridoma production. 
In recent years, there have been great advances in immunotherapy and gene therapy against such difficult targets as metastatic cancer, genetic defects, and acute viral infections. In many cases these advances have relied on the use of DNA and RNA. Today there are still no approved clinical uses of this technology because of the inability to deliver, safely and effectively, the DNA/RNA into the target cells. Cyto Pulse, a medical device company, has solved this problem with proprietary technologies using non-viral electric fields. After extensive preclinical studies these are now entering several human trials. The principal Cyto Pulse applications are the production of DNA therapeutic vaccines, delivery of DNA vaccines, modification of cell genomes to correct genetic defects, production of hybridoma cell vaccines by fusing immune system cells and tumor cells, and the production of hybridomas used to produce monoclonal antibodies. The annual markets for these treatments and vaccines are expected to be in the billions of dollars per year. The Cyto Pulse expertise and intellectual property is in waveform generators and electrodes for use in medicine and bioprocessing.
Management
Cyto Pulse Sciences, Inc. was founded by Richard Walters, MSEE, MBA and Alan King, DVM, Ph.D. Mr. Walters worked for the Department of Defense for 13 years, then founded a signal processing company which was eventually acquired by a NASDAQ company. Dr. King retired as a Lt Col. in the Army from the Infectious Diseases Branch at WRAIR. His expertise is in immunology and infectious diseases. Over the years he has spent time conducting infectious disease research in the Thailand, Peru, and Haiti.
Richard E. Walters, MSEE, MBA President and CEO 
Alan D. King, DVM, PhD Vice President 
John B. Burke, CPA, MBA Secretary and CFO 
Derin C. Walters, BSE, MBA (in process) Vice President 
Anna-Karin Roos, PhD Manager EU Operations
Facility
Cyto Pulse has a new 7000 square foot facility with a large bio laboratory, R&D electronics laboratory, QSR production facility and 200 square foot ISO 14644 compliant 'Class 5' clean room with sterilization apparatus. Cyto Pulse is located in the Cromwell Industrial Park adjacent to BWI Airport.